利用ISSR分子标记鉴定苦瓜杂交种纯度

[目的]采用 ISSR分子标记对苦瓜杂交一代品种秀玉1号纯度进行鉴定,为生产上提高苦瓜新品种纯度鉴定效率提供参考。[方法]利用ISSR技术和91条引物,对苦瓜品种秀玉1号及其亲本的DNA指纹进行分析。[结果]从91条引物中筛选出两条特征引物能同时扩增出F1代及其父母本的多态性条带,分别是ISSR-845和 ISSR-891,两个特异位点分别位于510和300 bp处。其中ISSR-891能够准确区分母本自交系与杂种 F1,经群体验证表明该标记与田间鉴定结果高度一致。[结论] ISSR技术具有准确、简便、高效和实用强等优点,可用于生产上苦瓜种子纯度的快速鉴定。     

瓠瓜高山栽培品种比较试验

比较分析了2种类型的6个瓠瓜品种在杭州高山露地栽培条件下的生育期、商品性和产量等性状指标。试验结果表明,长棒形品种以浙蒲6号和越蒲2号综合性状优良,在杭州高山地区具有极大推广前景。中棒形品种以浙蒲8号商品性较好,但产量相对偏低,可通过进一步研究其配套栽培技术来提高产量促进推广应用;越蒲1号目前不适合杭州市场消费习惯,但产量高,可作为外销储备品种。     

苦瓜3种不同遗传距离与产量杂种优势的关系研究

以14个苦瓜（白苦瓜、绿苦瓜和广西野生苦瓜）高世代自交系,配制出14个杂交组合,进行了苦瓜数量性状、蛋白质和ISSR分子标记3种遗传距离分析以及3种遗传距离与杂种优势之间关系的分析.结果表明：数量性状与蛋白质、ISSR分子标记遗传距离之间相关系数分别为0.8340和0.4532;蛋白质遗传距离与ISSR分子标记遗传距离的相关系数为0.5940;数量性状与蛋白质遗传距离与产量杂种优势之间均呈极显著直线回归关系;而ISSR分子标记遗传距离与产量杂种优势之间呈显著直线回归关系.     

Gene for broad, contiguous dark stripes in cocozelle squash (Cucurbita pepo)

Alternating intense (dark) and light stripes on the fruits are a fairly common feature of Cucurbita pepo, a highly variable species encompassing various kinds of pumpkins, squash, and gourds. The intense stripes can be narrow and broken or broad and contiguous. Narrow, broken intense striping is conferred by allele l-1 ^St, which is dominant to allele l-1 for non-striped, lightly colored fruits and recessive to allele L-1 for non-striped, intensely colored fruits. Cocozelle-type squash often exhibit broad, contiguous intense stripes. The inheritance of this characteristic was studied by transferring it from cocozelle squash and preparing nearly isogenic lines having broad-striped, narrow-striped, and non-striped light-colored fruits. In the broad-striped near-isogenic line, the intense stripes averaged 37% wider than in its narrow-striped counterpart. The broad-striped line was crossed with the narrow-striped line, the non-striped light-colored line, and the non-striped intense-colored `Fordhook Zucchini'. Broad striping was dominant to narrow striping and to non-striped light and conferred by a single gene; it was recessive to non-striped intense. The gene for broad striping was found to be allelic to that for narrow striping and to that for non-stripe dintense and is herein assigned the symbol l-1 ^BSt. Dominance relationships at the l-1 locus were observed to be L-1>l-1 ^BSt >l-1 ^St >l-1. This revised version was published online in July 2006 with corrections to the Cover Date.     

Effect of vegetable fibre on post prandial glycemia

Effects of feeding different levels of vegetable fibre to normal and non-insulin dependent diabetics were studied. Influences of a control diet (diet with no vegetable fibre) and a purified fibre diet (Isapgol) were compared to results achieved with different levels of several vegetables. A negative correlation between the level of fibre and blood glucose levels in terms of mean percent peak rise, AUC, and per cent glycemic response was shown. Bitter gourd was more effective than the leafy vegetables. Even though the trend in post prandial glucose levels was same for both normal and diabetic subjects, the metabolic response varied significantly between the two groups.     

墨绿色苦瓜新品种‘翠玉’

 ‘翠玉’为早中熟苦瓜一代杂交品种。果实长棒形, 长30～35 cm, 横径10～13 cm, 瓜肉厚1.0～1.5 cm, 单瓜质量500～700 g, 产量45～52.5 t/hm²。瓜皮墨绿色, 大刺瘤, 耐高温和耐低温能力强,抗白粉病和枯萎病。     

早熟丰产苦瓜长丰3号的选育

长丰3号苦瓜是汕头市白沙蔬菜原种研究所用C99-01A作母本、C01-03B作父本配制而成的杂交一代组合。果实长圆锥形,瓜长25～30cm,横径6～8cm,皮绿色,单瓜质量300～600g。中抗白粉病、枯萎病,耐热、耐寒、耐涝性强,适应性广。早熟,播种至初收春季为75天,夏秋直播后52天始收,每667m2产量3000～4000kg。     

葫芦砧木低温处理鉴定方法研究

对2个具有低温抗性差异的葫芦砧木A12、A05及西瓜TRM进行低温处理,试验结果表明,葫芦幼苗(0.43~0.71)的低温抗性显著高于西瓜(1.0),4℃条件下A12的耐低温性高于A05,6℃条件下相反;3叶期2个砧木间冷害指数差异显著,且叶绿素含量变化均达极显著,叶绿素含量变化程度与冷害指数具有显著正相关(R2=0.65),因此,叶绿素含量可以作为耐低温葫芦砧木材料选择的一个标准,且3叶1心期6℃为相对较好的低温处理条件。     

黑皮冬瓜嫁接砧木的筛选与鉴定

挑选南瓜砧、蒲瓜砧和葫芦砧共10个品种,以佛山市三水黑皮冬瓜为接穗,采用斜顶插接法嫁接,露地栽培管理,比较其产量和农艺性状。试验结果表明,DZ-01冬瓜砧、京欣砧三号和强势作为三水黑皮冬瓜的嫁接砧木,可有效降低枯萎病发病率,增产10%以上,商品性状得到明显改善,商品品质差异不显著,推广应用性强。     

墨绿色苦瓜新品种翠玉的选育

翠玉是以自交系0123为母本,0219为父本配制成的苦瓜一代杂种.中早熟,果实长棒形,纵径30～35 cm,横径10～13 cm,瓜肉厚1.0～1.5 cm,单瓜质量500～700g,产量2 500～3000kg·(667 m2)-1,皮墨绿色,大刺瘤,对白粉病和枯萎病的抗性强于对照翠妃,适宜南方春秋种植.